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Image Search Results
Journal: Journal of Advanced Research
Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling
doi: 10.1016/j.jare.2025.07.017
Figure Lengend Snippet: HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) and claudin-1 (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Membranes were blocked using 5 % skim milk at room temperature for 1 h and subsequently incubated at 4 °C overnight with primary antibodies against FXR (1:1000, Immunoway, YN2161), zonula occludens-1 (ZO-1) (1:1000, Affinity, AF5145), Mucin-2 (1:1000, Abcam, ab272692), occludin (1:1000, Proteintech, 27260–1-AP),
Techniques: Concentration Assay, Expressing, Western Blot, Immunohistochemical staining, Staining, Immunofluorescence, Fluorescence, Transmission Assay, Electron Microscopy