Journal: Journal of Advanced Research

Article Title: Hyodeoxycholic acid relieves neuropathic pain by activating farnesoid X receptor signaling

doi: 10.1016/j.jare.2025.07.017

Figure Lengend Snippet: HDCA alleviates neuropathic pain and enhances intestinal barrier function. (A) The concentration of total bile acids in fecal samples (n = 6). (B) The expression of HDCA in fecal samples (n = 6). (C) Schedule for the management of HDCA. (D, E) PWT and PWL of mice following HDCA treatment (n = 6). Representative immunoblots (F) and relative expression levels of FXR mRNA (G) and FXR protein (H). (I) Immunohistochemical staining of FXR + cells and Alcian Blue staining in the distal ileum (scale bar, 100 μm, n = 6). (J) Quantitative analysis of FXR + cells. The relative expression levels of ZO-1 (K), occludin (L) and claudin-1 (M) protein in distal ileum (n = 3). (N) Representative immunoblots and relative expression levels of Mucin-2 (P) (n = 3). (O) Quantitative analysis levels of the number of goblet cells in the distal ileum (n = 6). (Q, R) Immunofluorescence staining and mean fluorescence intensity of Mucin-2 (scale, 100 μm, n = 3). (S) Transmission electron microscopy examination of the distal ileum. (scale, 1.0 μm). * P < 0.05, ** P < 0.01 by unpaired Student's t‐test (A and B). * P < 0.05, ** P < 0.01 *** P < 0.001, # P < 0.05, ## P < 0.01 by Two-way repeated ANOVA with post hoc Bonferroni’s test (D and E), * P < 0.05, ** P < 0.01, *** P < 0.001 by One-way ANOVA with post hoc Tukey's test (G, H, J–M, O–Q). Data is presented as mean ± SEM. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Membranes were blocked using 5 % skim milk at room temperature for 1 h and subsequently incubated at 4 °C overnight with primary antibodies against FXR (1:1000, Immunoway, YN2161), zonula occludens-1 (ZO-1) (1:1000, Affinity, AF5145), Mucin-2 (1:1000, Abcam, ab272692), occludin (1:1000, Proteintech, 27260–1-AP), claudin-1 (1:1000, WL, WL03073), MMP-2 (1:1000, Immunoway, YT2798), MMP-9 (1:1000, Immunoway, YT1892), PPAR-γ (1:1000, Proteintech, 16643-1-AP), NOD-like receptor protein-3 (NLRP3) (1:1000, Immunoway, YT5382), interleukin-6 (IL-6) (1:1000, WL, WL02841), IL-18 (1:1000, WL, WL01127), tumor necrosis factor-alpha (TNF-α) (1:1000, WL, WL01581), IL-10 (1:1000, WL, WL03088), FGF15 (1:500, Santacruz, sc-514647), TGR5 (1:1000, Abcam, ab72608), BSEP (1:1000, Abcam, ab155421), CYP7α1 (1:1000, Abcam, ab65596) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, 60004-1-Ig).

Techniques: Concentration Assay, Expressing, Western Blot, Immunohistochemical staining, Staining, Immunofluorescence, Fluorescence, Transmission Assay, Electron Microscopy

Journal: iScience

Article Title: Butyrate ameliorates doxorubicin-induced heart failure by inhibiting cardiomyocyte ferroptosis through the gut-heart axis

doi: 10.1016/j.isci.2026.114754

Figure Lengend Snippet: NaB reduced bacterial translocation and LPS levels by enhancing the barrier function of the colonic and cardiac tissues (A) Histopathological damage of colonic tissue in the DOX+NaB, DOX +NaCl group by Hematoxylin and Eosin (H&E) staining (scale bars, 50 μm). (B and C) Representative images, quantitative analysis of Claudin-1 (B) and ZO-1 (C) of colonic tissue in the DOX+NaB, DOX+NaCl group by immunofluorescence (scale bars, 50 μm). (D) Representative images of goblet cell changes of colonic tissue in the DOX+NaB, DOX+NaCl group by AB-PAS staining (scale bars, 50 μm). (E) The mRNA expression levels of Muc2 and Tff3 in the colonic tissue via qRT-PCR assay in the DOX+NaB and DOX+NaCl groups. (F) Serum and cardiac tissue LPS concentrations in the DOX+NaB and DOX+NaCl groups via ELISA. (G and H) Representative images (G), quantitative analysis (H) of EUB338 bacterial probe count in cardiac tissue of the DOX+NaB and DOX+NaCl groups by FISH (scale bars, 20 μm). (I) The Pearson’s correlation analysis of EUB338 probe count and LVEF decrease rate in the DOX+NaB and DOX+NaCl groups. Statistical significance was determined using the simple linear regression of Correlation. (J) Representative images and quantitative analysis of connexin-43 and N-cadherin in cardiac tissue of the DOX+ NaB, DOX + NaCl group by immunofluorescence (scale bars, 50 μm). DOX+NaB and DOX+NaCl groups, n = 8 per group. Error bars represent the mean ± SEM. For normally distributed data with homogeneous variances, statistical analyses were performed using Student’s t tests for two groups and one-way ANOVA for multiple comparisons. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: Rabbit Claudin 1 Polyclonal antibody , proteintech , Cat# 28674-1-AP; RRID: AB_2881190.

Techniques: Translocation Assay, Staining, Immunofluorescence, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay