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Image Search Results
Journal: Frontiers in pharmacology
Article Title: Andrographolide Inhibits Cholangiocarcinoma Cell Migration by Down-Regulation of Claudin-1 via the p-38 Signaling Pathway.
doi: 10.3389/fphar.2019.00827
Figure Lengend Snippet: FIGURE 4 | Inhibitory effect of andrographolide on cell migration mediated by claudin-1. The RNA interference of claudin-1 was used to knock down the claudin-1 gene in KKU-M213 cells. KKU-M213 were treated with 0 μM (1% DMSO), 50 μM of andrographolide, scramble, and si-claudin-1 prior to harvesting RNA and cellular proteins for the detection of claudin-1. (A) The expression of claudin-1 mRNA from real-time RT-PCR; (B) The level of claudin-1 protein from Western blot analysis. Actin was used as internal standard. (C) With those treatments, KKU-M213 was scratched to make a wound and photographed at 0 and 12 h to measure the wound length for migration index analysis. (D) The results of bar graph are represented by the average of relative intensity compared with that of control. Data were derived from three independent experiments and are presented as mean ± SE, *p < 0.05, **p < 0.01.
Article Snippet:
Techniques: Migration, Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Control, Derivative Assay
Journal: Food Science and Biotechnology
Article Title: Promoting effect of the Maillard reaction products produced during the stir-frying process of Hordei Fructus Germinatus on the intestinal absorption of active ingredients in Hordei Fructus Germinatus
doi: 10.1007/s10068-021-00911-1
Figure Lengend Snippet: Effects of MRPs on the mRNA and protein expression levels of claudin-1 and ZO-1 in duodenal tissues. (A) The mRNA expression levels of claudin-1. (B) The mRNA expression levels of ZO-1. (C) The protein expression levels of claudin-1. (D) The protein expression levels of ZO-1. The mRNA levels of claudin-1and ZO-1 were quantified with β-actin as reference gene. The protein expression levels of claudin-1and ZO-1 were quantified with β-actin as the internal control. Values are mean ± SD (n = 6). *P < 0.05, compared with the control group; △P < 0.05, compared with the S-R-HGF group
Article Snippet: Rabbit polyclonal anti-ZO-1 antibody and
Techniques: Expressing